Gas & Liquid Chromatography & Troubleshooting

Gas & Liquid Chromatography & Troubleshooting

Date & Location: (22-26/Jan) Kuala Lumpur

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Course Overview


Gas & Liquid Chromatography and Troubleshooting is a comprehensive seminar covering the following areas:

  • Sample preparation
  • Sample introduction
  • Sample separation
  • Columns
  • Detectors
  • Data acquisition
  • Troubleshooting
  • Reporting


Program Objectives:

This course is designed to give the participants the capability to:

  • Gain the basic theoretical aspects of Gas Chromatography
  • Communicate practical information, capabilities and limitations of Gas & Liquid Chromatography
  • Gain confidence on the analysis technique, troubleshooting and analytical results evaluation

Who Should Attend?

This seminar is ideal for lab and quality professionals that do not have formal training or experience in gas chromatography or those that want to refresh their current knowledge of gas chromatography. It is also suitable for Environmental Quality Personnel.


Program Outline:


Introduction to Chromatography

  • The History of Chromatography – GC & LC
  • Overview of GC & LC Chromatography
  • The Modern Chromatography
  • Liquid Chromatography – The Development Process
  • Factors Controlling Retention
  • Molecular Forces and Chromatographic Selectivity
  • Effects of Stationary Phase Loading on the Performance of a Chromatographic System
  • Chromatography Nomenclature


Chromatography Basics

  • Basic Chromatography
  • Sample Introduction
  • The role of sample introduction and injection ports in GC operations
  • Injection ports maintenance and its impact on GC performance
  • Columns
  • The role of columns in GC operations
  • Column selection & maintenance
  • How columns can impact GC performance


GC Operation 

  • Peak Dispersion in a Chromatographic Column
  • Detector Selection
  • The role of Detectors in GC operations
  • Detector maintenance
  • How detectors can impact GC performance
  • Setup and GC operation, basic steps
  • Preparation for operation
  • Sampling Techniques


Data Acquisition and Calibration

  • Calibration
  • Data Acquisition and Processing System
  • Calibration linked to GC performance


Applications and Troubleshooting

  • Chromatography Applications
  • Method Development
  • Gas Chromatography
  • Liquid Chromatography
  • ISO17025 Accreditation Basics
  • Laboratory Management & Troubleshooting